目的研究并建立不同产地铁皮石斛药材的指纹图谱。方法采用ZORBAXSB-C18(4。6mm×250mm，5μm)为色谱柱，流动相为乙腈-0。4%磷酸溶液梯度洗脱，检测波长为230nm，流速:1。0mL·min-1，柱温:30℃，建立了铁皮石斛药材的指纹图谱，并进行了相似度的计算。结果该方法使铁皮石斛中各成分得到较好的分离，并根据检测结果确定了26个共有峰。结论该指纹图谱方法准确可靠，重现性好，为铁皮石斛药材的质量控制提供了方法依据。
Abstract
OBJECTIVE To study and establish the fingerprint of Dendrobium officinale from different origins. METHODS HPLC was conducted on Agilent ZORBAX SB-C18 column (4.6 mm×250 mm, 5 μm). Acetonitrile-0.4% phosphoric acid aqueous solution was employed as mobile phase with gradient elution, the detection wavelength was set at 230 nm, flow rate was 1.0 mL·min-1,and the column temperature was maintained at 30 ℃. RESULTS The chemical constituents of Dendrobium officinale were well separated. The fingerprint of Dendrobium officinale was established and the similarities were calculated, among which 26 fingerprint peaks in common were confirmed. CONCLUSION The method is accurate, reliable, and reproducible, which provides a reference for the quality control of Dendrobium officinale.
关键词
铁皮石斛 /
指纹图谱 /
相似度
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Key words
Dendrobium officinale /
fingerprint /
similarity
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中图分类号：
R284
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参考文献
Ch.P(2010) VolⅠ(中国药典 2010年版.一部).2010:265-266. YANG Y L,LAI P F,JIANG S P.Advances in research of Dendrobium officinale.J Shandong Univ Tradit Chin Med(山东中医药大学学报),2008,32(1):82-85. SHAO H,ZHANG L Q,LI J M,et al.Advances in research of Dendrobium officinale.Chin Tradit Herb Drugs(中草药),2004,35(1):109-112. LI L,DENG X L,ZHAO X B,et al.Advances in studies on chemical constituents in Dendrobium candidum and their pharmacological effects.Antitumor Pharmacy(肿瘤药学),2011,1(2):90-95. SONG J Y,GUO S X,XIAO P G.Dendrobium plant research in the past 10 years.Chin Pharm J(中国药学杂志),2004,39(10):725-727. ZHENG S J,LU G Y,CHEN S H,et al.Determination of sugar content of Dendrobium candidum and several common counterfeit drugs in the market .Chin Arch Tradit Chin Med(中华中医药学刊),2012,30(1):146-148. NIE L,LUO G A,CAO J,et al.Pattern recognition applied to the fingerprint of traditional Chinese medicine characterized by two dimension information data .Acta Pharm Sin(药学学报),2004,39(2):136-139. LIU R X,WANG X,BI K S,et al.Chromatographic fingerprint of Dalbergia odorifera and its application in the identification of the crude drugs of different origins.Acta Pharm Sin(药学学报),2005,40(11):1008-1012. LIU Y S,MENG Q H,JIANG S M,et al.Similarity system theory to evaluate similarity of chromatographic fingerprints of traditional Chinese medicine.Chin J Chromatogr(色谱),2005,23(2):158-163.
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脚注
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基金
中国普洱茶研究院委托项目；云南省科技厅社会发展科技计划 (Y237321201)
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